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thesis last version revised(N)10

Alyssum homolocarpum based films incorporated with Ajowan(Trachyspermum ammi L.) essential oil at 10, 20 and 30% v/v were prepared to examine their antibacterial, antioxidant, physical, thermal, and mechanical properties. Extraction of the seed gum was determined using the method employed by Cui et al. (1994) with some modification. Fordistillation of Ajowan seeds, the method recommended in British Pharmacopoeia was used.  The physical and mechanical properties were determined in accordance with the ASTM standard methods.Addition of Ajowan essential oil affected the oxygen and water vapor permeability of the films. Incorporating AEO, increased color parameters and decreased lightness of films. A decrease of young modulus (YM) and tensile strength (TS) accompanied with an increase in elongation at break (% EB) was observed with increasing oil concentration. These films exhibited some antioxidant activity, which was significantly improved by the addition of the Ajowan essential oil. This incorporation influenced antimicrobial, antioxidant, physical, mechanical, barrier, microstructure and color properties of the films. The films prepared with Ajowan essential oil exhibited highest inhibition against E. coli and were more effective against Gram-negative bacteria (E.coli) than Gram-possitive bacteria (S. aureus). The results pointed out that the incorporation of ajowan essential oil as a natural antibacterial agent has potential for using the developed film as an active packaging.

ACKNOWLEDGMENTS. II

Table of Contents. III

List of figures. VII

List of tables. VIII

Abstract.. IX

Chapter1: Introduction.. 10

1-1-Background. 11

1-1-1-History. 14

1-1-2- Application and Preparation of Edible Films. 15

1-2-Significant of study. 16

1-2-1-Structural matrix of films. 16

1-2-2-Edible films as active packages. 18

1-3-Commercial and regulatory of Edible film and coating. 19

1-4-Objectives. 21

1-5-Questions. 22

1-6-Limitations. 22

1-7- Research flowchart. 22

Chapter 2: Literature review… 24

2-1-Classification of films. 25

2-2-Hydrocolloids Used to Constitute Edible Films and Coatings. 25

2-2-1-Polysaccharides. 26

2-2-2- Cellulose and Derivatives. 26

2-2-3- Starches and Derivatives. 28

2-2-4- Seaweed Extracts. 29

2-2-5- Gums. 31

2-2-6- pectins. 32

2-2-7- Chitosan. 33

2-2-8- Proteins. 34

2-2-9- Blends. 35

2-3– Alyssum homolocarpum as film Hydrocolloid. 38

2-4- What are gums and mucilages?. 41

2-4-1- Classification of gums and mucilages. 42

2-4-1-1 According to the charge. 42

2-4-1-2 According to the source. 42

2-4-1-3- Semi-synthetic. 43

2-4-1-4- According to shape. 43

2-4-1-4- According to monomeric units in chemical structure. 43

2-5- Antimicrobials Used in Edible Films and Coatings. 44

2-5-1- Organic Acids. 45

2-5-2- Acidulants. 45

2-5-3- Weak Lipophilic Acids. 48

2-5-4- Nisin. 50

2-5-5-Antimicrobials from Herbs and Spices. 52

2-6- Trachyspermum ammi L. as Antimicrobial agent. 56

2-7- Film preparation and properties. 57

2-8- Recent studies. 60

2-9-Biopolymer films characterization. 63

2-9-1- Mechanical properties. 63

2-9-1-1-Elongation. 64

2-9-1-2- Tensile stress. 64

2-9-2-Barrier properties. 64

2-9-2-1- WVP. 65

2-9-2-2-Oxygen permeability (OP) 65

2-9-3-FTIR investigation for chemical interaction. 66

2-9-4-Color measurement 66

2-9-5-Thickness of film.. 66

2-9-6- Solubility. 67

2-9-7-Equilibrium adsorption diagrams. 67

2-9-8-Free Radical Scavenging Activity. 68

Chapter 3: Materials and methodes. 69

3-1-Materials. 70

3-2-Gum Extraction. 71

3-3-Distillation. 72

3-4-GC/MS analysis. 72

3-5- Film Preparation. 73

3-6- Thickness of film… 74

3-7- Mechanical properties. 75

3-8-Color measurement. 76

3-9-Water vapor permeability (WVP) 77

3-10-Solubility of films. 78

3-11- Sorption isotherms. 79

3-12- Oxygen permeability (OP) 80

3-13-Antimicrobial efficiency. 81

3-14-DPPH radical-scavenging activity. 82

3-15 – FTIR investigation for chemical interaction. 83

3-16 – Water absorption capacity (WAC) 83

3-17 -Statistical analysis. 84

Chapter 4: Results and Discussion.. 85

4-1- Films Thickness and Appearance. 86

4-2- Mechanical Properties of Films. 87

4-3- Colorimetric Characteristics. 88

4-4-Barier properties. 89

4-4-1- Water Vapor Permeability of Films. 89

4-4-2- oxygen permeabilityof Films. 90

4-5- Solubility and Water Absorption Capacity. 90

4-6- DPPH radical-scavenging activity. 91

4-7- Antimicrobial efficiency. 92

4-8- FTIR investigation for chemical interaction. 95

4-9- Sorption Isotherm of the Films. 96

Chapter 5: Conclusion and Suggestion.. 97

5-1-Conclusion. 98

5-2- Suggestions. 99

Refrences. 100

چكيدهفارسی.. 124

 

List of figures

Figure 1. 1: Research flochart 23

Figure 2. 1: Alyssum homolocarpum plant 41

Figure 2. 2: Ajowan (Trachyspermum ammi L. synonym: Carum copticum Benth. & Hook) plant 57

Figure 3. 1: Qudum-e Shirazi (Alyssum homolocarpum) seeds. 70

Figure 3. 2: Ajowan( Trachyspermum ammi L. synonym: Carum copticum Benth. & Hook) seeds  70

Figure 3. 3: Gum extraction. 71

Figure 3. 4: Extarcted Gum.. 71

Figure 3. 5: Distillation of Ajowan (Trachyspermum ammi L.) seeds. 72

Figure 3. 6: GC-MS analyzer 73

Figure 3. 7: Film formation. 74

Figure 3. 8: Water vapour permeability test 77

Figure 3. 9: Dynamic shake flask method. 82

Figure 3. 10: DPPH scavenging activity. 83

Figure 4. 1: DPPH radical-scavenging activity of Alyssum homolocarpum based films incorporated with various Ajowan essential oil (AEO) concentrations. 92

Figure 4. 2: Growth curves of E. coli in the presence of Alyssum homolocarpum based films incorporated with various Ajowan essential oil (AEO) concentrations. 94

Figure 4. 3: Growth curves of S.aureous in the presence of Alyssum homolocarpum based films incorporated with various Ajowan essential oil (AEO) concentrations. 94

Figure 4. 4: Fourier transform infrared spectra ofAlyssum homolocarpum based films incorporated with various Ajowan essential oil (AEO) concentrations. 95

Figure 4. 5: Moisture sorption isotherm for Alyssum homolocarpum based films incorporated with various Ajowan essential oil (AEO) concentrations at 25 °C. 96

List of tables

Table 3. 1: Water activity aw [ASTM, 2007] 80

Table 4. 1: Thickness of Alyssum homolocarpum based films incorporated with various Ajowan essential oil (AEO) concentrations. 86

Table 4. 2: Mechanical properties of Alyssum homolocarpum based films incorporated with various Ajowan essential oil (AEO) concentrations. 88

Table 4. 3: Colorimetric parameters of Alyssum homolocarpum based films incorporated with various Ajowan essential oil (AEO) concentrations. 88

Table 4. 4: Barier properties of Alyssum homolocarpum based films incorporated with various Ajowan essential oil (AEO) concentrations. 90

Table 4. 5:  Solubility and Water Absorption Capacity of Alyssum homolocarpum based films incorporated with various Ajowan essential oil (AEO) concentrations. 91

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