فهرست مطالب
عنوان
مقدمه………………………………………………………………………………………………………………………………………………. 2
فصل اول: مروری بر تحقیقات انجام شده
1- شتر مرغ Ostrich. 4
1-1- رده‌بندی شتر مرغ. 4
1-1-2- بیولوژی شترمرغ.. 5
1-1-3- آناتومی دستگاه تولید مثل شتر مرغ.. 6
1-1-4- رفتارهای جنسی در شترمرغ.. 7
1-2- پرورش صنعتی شترمرغ. 10
1-2-1- موارد قابل ملاحظه در جوجه‌کشی.. 12
1-2-1-1- ورود تخم به جوجه‌کشی.. 12
1-2-1-2- ضدعفونی تخم‌ها 13
1-2-1-3- انبار کردن تخم‌ها 14
1-2-1-4- انتخاب تخم برای جوجه‌کشی.. 15
1-2-2- دستگاه انکوباتور. 16
1-2-3- دستگاه هچر. 17
1-3- مباني نظري تحقيق.. 20
1-3-1- تکوین جنین پرندگان.. 20
1-3-2- مرحله‌بندی تکوین جنین پرندگان.. 21
1-3-3- تکوین جنین شتر مرغ.. 23
1-4- تعیین جنسیت درجانوران. 27
1-4-1- تعیین جنسیت اولیه توسط ژن.. 27
1-4-2- تعيين جنسيت ثانويه: تنظيم هورموني فنوتيپ جنسي و تمایز گناد 28
1-4-2-1- تستوسترون و دي هيدروتستوسترون.. 29
1-4-2-2- هورمون ضد مولر. 29
1-4-2-3- استروژن.. 30
1-5- تعيين جنسيت در پرندگان. 30
1-5-1- تعیین جنسیت اولیه توسط ژن‌ها 30
1-5-2- هورمون‌ها وتمایزجنسی پرندگان.. 34
1-5-2-1- استروژن.. 34
1-5-2-2- هرمون ضد مولر. 36
1-6- تکوین گناد پرنده 37
1-6-1- عدم تقارن گناد‌ها در پرندگان.. 40
1-6-1-1- عدم تقارن مولکولی.. 41
1-7- تغییرات مورفومتریک تخمدان در طی روند تکوین جوجه. 44
1-7-1- تغییرات ماکروسکوپی (آناتومی) 44
1-7-2- تغییرات میکروسکوپی (بافت شناسی) 45
1-8- سلول‌های زایا 46
1-8-1- اختصاصی شدن سلول‌های زایای اولیه. 49
1-8-2- شناسایی سلول‌های زایا 51
1-8-3- تقسیم میوز در سلول‌های زایا 54
1-8-4- مجموعه سیناپتو نمال synaptonemal complexes (SCs) 55
1-9- استریولوژی.. 57
1-9-1- نمونه‌گیری ایزوتروپیک و تصادفی یکنواخت… 58
1-9-2- آماده‌سازی نمونه‌های بافتی برای استریولوژی.. 60
1-9-3- تخمین حجم 61
1-9-4- شمارش سلول.. 64
فصل دوم: مواد و روش‌ها
2-1- مواد ووسایل مورد نیاز. 67
2-2- تهیه و انکوباسیون تخم شترمرغ. 69
2-3- نمونهبرداری.. 70
2-3-1- نمونهبرداری ازتخمدان رویان 20 روزه 71
2-3-2- نمونهبرداری ازتخمدان رویان 26 روزه 72
2-3-3- نمونهبرداری از تخمدان رویان 30 و 36 روزه 73
2-3-4- نمونهبرداری از تخمدان جوجه‌شترمرغ تازه متولد شده 73
2-3-5- تعیین جنسیت جوجه شتر مرغ.. 74
2-4- ثابت‌سازی نمونه‌های بافتی.. 77
2-4-1- ثابت‌سازی نمونه‌های بافتی برای ایمونوهیستوشیمی.. 77
2-4-2- ثابت‌سازی نمونه‌ها برای تهیه برش نیمه‌نازک و فوق‌نازک… 77
2-4-3- ثابت‌سازی نمونه‌ها با محلول بوئن.. 78
2-4-4- ثابت‌سازی نمونه‌ها با محلول فرمالین بافر. 79
2-5- آماده‌سازی نمونه‌های بافتی.. 79
2-5-1- آماده‌سازی نمونه‌ها برای تهیه مقاطع معمولی.. 79
2-5-2- آماده‌سازی نمونه‌ها جهت تهیه مقاطع نیمه‌نازک و فوق‌نازک (مطالعات فراساختاری با کمک میکروسکوپ الکترونی عبوری) 80
2-6- برش معمولی.. 81
2-7- برش نیمه‌نازک….. 82
2-8- برش نازک….. 83
2-9- رنگآمیزی مقاطع بافتی و مونته کردن. 83
2-9-1- رنگآمیزی هماتوکسیلین و ائوزین.. 84
2-9-2- رنگآمیزی تریکروم ماسون سبز. 85
2-9-3- رنگآمیزی آلسین‌بلو. 86
2-9-4- رنگآمیزی پریودیک اسید شیف یا PAS. 87
2-9-5- رنگآمیزی پیگ ایندیگو کارمین.. 88
2-9-6- رنگ آمیزی برش‌های فوق نازک جهت مطالعه میکروسکوپ الکترونی عبوری.. 88
2-9-7- رنگ آمیزی ایمونوهیستوشیمی جهت تشخیص شروع میوز. 89
2-10- استریولوژی.. 91
2-10-1- جهت تعیین حجم 91
2-10-2- شمارش سلول‌های زایا 92
فصل سوم: نتايج
3-1- مشاهدات ماکروسکوپی تخمدان‌ها در جنین و جوجه تازه متولد شده شتر مرغ. 94
3-2- مورفومتری تخمدان چپ و راست در جنین و جوجه تازه متولد شده شتر مرغ. 97
3-3- مشاهدات بافت شناسی تخمدان‌ها در جنین و جوجه تازه متولد شده شتر مرغ. 98
3-3-1- بافت شناسی تخمدان در جنین 18 روزه شتر مرغ.. 98
3-3-2- بافت‌شناسی تخمدان چپ در جنین 20 روزه شتر مرغ.. 99
3-3-3- بررسی بافت شناسی تخمدان چپ جنین 26 روزه شتر مرغ.. 104
3-3-4- بررسی بافت شناسی تخمدان چپ جنین 30 روزه 105
3-3-5- بررسی بافت شناسی تخمدان چپ جنین 36 روزه شتر مرغ.. 106
3-3-6- بررسی بافت شناسی تخمدان چپ جوجه تازه متولد شده شتر مرغ.. 107
3-3-7- بافت شناسی تخمدان راست در جنین و جوجه تازه متولد شده شترمرغ. 110
3-4- مطالعه فراساختار تخمدان چپ در جنین و جوجه شتر مرغ. 112
3-4-1- مطالعه فراساختار تخمدان چپ در رویان 20 روزه شتر مرغ.. 112
3-4-2- بررسی فراساختار تخمدان چپ جنین 26 روزه شتر مرغ.. 115
3-4-3- بررسی فراساختار تخمدان چپ جنین 36 روزه شتر مرغ.. 117
3-4-4- مطالعه فراساختار تخمدان چپ در جوجه تازه متولد شده شترمرغ.. 118
3-4-5- مطالعه فراساختارتخمدان راست در جنین 36 روزه شتر مرغ.. 121
3-5- شناسایی پروتئین سیناپتونمال کمپلکس در سلول‌های زایای پیش میوزی به روش ایمونوهیستوشیمی 123
3-6- نتایج مطالعات استریولوژیکی تخمدان چپ در جنین 20 روزه و جوجه تازه متولد شده 126
3-6-1- تخمین حجم تخمدان چپ در جنین 20 روزه شتر مرغ.. 126
3-6-2- تخمین حجم تخمدان چپ جوجه تازه متولد شده شترمرغ.. 127
3-6-3- شمارش تعداد سلول‌های زایا در کورتکس تخمدان چپ جنین 20 روزه و جوجه تازه متولد شده شتر مرغ 128
فصل چهارم: بحث، نتيجه‌گيري و پيشنهادها
4-1- تکوین آناتومی تخمدان‌ها 130
4-2- تکوین بافت شناختی تخمدان‌ها 131
4-3- مطالعه مورفومتریک….. 133
4-4- مطالعه فراساختار تخمدان جنین و جوجه شتر مرغ. 137
4-5- بررسی حضور مجموعه سیناپتونمال در تخمدان جنین و جوجه شترمرغ. 140
منابع
منابع. 143
فهرست جدولها
عنوان
جدول 1- 1. رده‌بندي شترمرغ.. 4
جدول 1- 2. مقایسه مراحل مهاجرت PGCs در پرندگان و پستانداران.. 49
جدول 2- 1. مواد و لوازم لازم برای مراحل مختلف PCR.. 75
جدول 2- 2. مراحل روش PLT. 80
جدول 3- 1. تغییرات طول در تخمدان راست و چپ در جنین و جوجه شترمرغ.. 97
جدول 3- 2. تغییرات عرض تخمدان راست وچپ در جنین و جوجه شترمرغ.. 97
جدول 3- 3. نتایج تخمین حجم کل تخمدان چپ و حجم‌های کورتکس، مدولا وحفرات لاکونار در جنین 20 روزه (µl) 126
جدول 3- 4. نتایج تخمین حجم کل تخمدان چپ و حجم‌های کورتکس، مدولا وحفرات لاکونار در جوجه تازه متولد شده (µl) 127
جدول 3- 5. نتایج تخمین تعداد کل سلول‌های زایا در جنین 20 روزه 128
جدول 3- 6. نتایج تخمین تعداد کل سلول‌های زایا در جوجه یک روزه 128
فهرست شکلها
عنوان
شکل 1- 1. شترمرغ نر و ماده در مزرعه پرورش شتر مرغ شاندیز مشهد 6
شکل 1- 2. مرفولوژی تخمدان شتر مرغ بالغ. 7
شکل 1- 3. رفتار جفت گیری شتر مرغ مزرعه پرورش شتر مرغ شاندیز مشهد 9
شکل 1- 4. مزرعه پرورش شترمرغ شاندیز مشهد 12
شکل 1- 5. نمایی از یک سالن نگهداری تخم شترمرغ.. 13
شکل 1- 6. دستگاه انکوباسیون مزرعه پرورش شترمرغ شاندیز مشهد 18
شکل 1- 7. کندل کردن تخم در مزرعه پرورش شترمرغ شاندیز مشهد 19
شکل 1- 8. دستگاه هچر مزرعه پرورش شترمرغ شاندیز مشهد 19
شکل 1- 9. جوجه‌های تازه متولد شده مزرعه پرورش شترمرغ شاندیز مشهد 20
شکل 1- 10. تصویر رویان شترمرغ در روزهای زوج 4 تا 22.. 25
شکل 1- 11. تصویر رویان شترمرغ در روزهای زوج 24 تا 42.. 25
شکل 1- 12. مقایسه اندازه رویان جوجه و امو. 26
شکل 1- 13. تعیین جنسیت پرندگان.. 34
شکل 1- 14. تعیین جنسیت و تمایز جنسیتی در پرنده 36
شکل 1- 15. استقرار سلول های زایای اولیه در گناد 48
شکل 1- 16. نمونه برداری تصادفی ساده در مقابل نمونه برداری سیستماتیک تصادفی یکنواخت 60
شکل 2- 1. انکوباسیون تخم‌شترمرغ در آزمایشگاه بافت‌شناسي دانشكده دامپزشكي.. 70
شکل 2- 2. نمایش مراحل مختلف نمونه برداری از جنین شترمرغ.. 72
شکل 2- 3. نمایش مراحل نمونهبرداری از جوجه شتر مرغ تازه متولد شده 74
شکل 2- 4. تصویر محصول‌های PCR رانده شده بر روی ژل آگارز. 76
شکل 3- 1. موقعیت تخمدان‌ها در جنین بیست روزه شترمرغ. 95
شکل 3- 2. وضعیت تخمدان چپ و راست در جنین 26 روزه شترمرغ.. 95
شکل 3- 3. تخمدان چپ و راست در جنین 36 روزه شترمرغ.. 96
شکل 3- 4. وضعیت تخمدان چپ و راست در جوجه تازه متولد شده شترمرغ.. 96
شکل 3- 5. تخمدان در جنین 18 روزه که با هماتوکسیلن- ائوزین رنگ آمیزی شده. 99
شکل 3- 6. تصویر میکروسکوپی از تخمدان چپ جنین 20 روزه که با ماسون تری کروم رنگ شده است. 101
شکل 3- 7. تصویر میکروسکوپی از تخمدان چپ 20 روزه که با رنگ آلسین- پاس رنگ شده است.. 101
شکل 3- 8. تصویر میکروسکوپی از کورتکس تخمدان چپ 20 روزه که با رنگ آلسیان – پاس رنگ شده است. 102
شکل 3- 9. تصویر میکروسکوپی از مدولا تخمدان چپ 20 روزه که با رنگ آلسیان- پاس رنگ شده است. 102
شکل 3- 10. (A) تصویر میکروسکوپی برش نیمه نازک از تخمدان چپ جنین 20 روزه که با آبی تولوئیدین رنگ شده است. 103
شکل 3- 11. (A) تصویر میکروسکوپی تخمدان چپ جنین 26 روزه شتر مرغ که با تکنیک پاس رنگ شده است. 104
شکل 3- 12. تصویر میکروسکوپی برش نیمه نازک از تخمدان چپ جنین 30 روزه که با آبی تولوئیدین رنگ شده است. 105
شکل 3- 13. (A) تصویر میکروسکوپی برش نیمه نازک رزینی از تخمدان چپ جنین 36 روزه که با آبی تولوئیدین رنگ شده است. 106
شکل 3- 14. تصویر میکروسکوپی تخمدان چپ جوجه تازه متولد شده که با ماسون تری کروم رنگ شده است. 108
شکل 3- 15. تصویر میکروسکوپی تخمدان چپ جوجه تازه متولد شده که با تکنیک پاس رنگ شده است. 109
شکل 3- 16. تصویر میکروسکوپی تخمدان چپ جوجه تازه متولد شده که با رنگ پیگ ایندیگو کارمین رنگ شده است. 109
شکل 3- 17. تصویر میکروسکوپی برش نیمه نازک از تخمدان چپ جوجه تازه متولد شده که با آبی تولوئیدین رنگ شده است… 110
شکل 3- 18. تصویر میکروسکوپی از تخمدان راست در جنین شتر مرغ.. 111
شکل 3- 19. تصویر میکروسکوپی از تخمدان راست در جوجه تازه متولد شده شتر مرغ.. 111
شکل 3- 20. تصویر میکروسکوپ الکترونی گذاره از کورتکس تخمدان چپ جنین 20روزه شتر مرغ. 113
شکل 3- 21. تصویر میکروسکوپ الکترونی گذاره از کورتکس تخمدان چپ جنین 20روزه شتر مرغ. 114
شکل 3- 22. تصویر میکروسکوپ الکترونی گذاره از کورتکس تخمدان چپ جنین 26روزه شتر مرغ. 116
شکل 3- 23. تصویر میکروسکوپ الکترونی گذاره از کورتکس تخمدان چپ جنین شتر مرغ 36 روزه. 117
شکل 3- 24. تصویر میکروسکوپ الکترونی گذاره از سلول زایا(GC) درکورتکس تخمدان چپ جوجه تازه متولد شده شتر مرغ. 119
شکل 3- 25. تصویر میکروسکوپ الکترونی گذاره از کورتکس تخمدان چپ جوجه تازه متولد شده شتر مرغ که ارتباط بین دو سلول زایا را نشان می‌دهد 120
شکل 3- 26. تصویر میکروسکوپ الکترونی گذاره از مدیا درتخمدان راست جنین 36 روزه شتر مرغ. 121
شکل 3- 27. تصویر میکروسکوپ الکترونی گذاره از تخمدان راست جنین 36 روزه شتر مرغ. 122
شکل 3- 28. تصویر میکروسکوپ الکترونی گذاره از مدیا در تخمدان راست جنین 26 روزه شتر مرغ. 122
شکل 3- 29. تصویرمیکروسکوپی بیضه موش سوری بالغ به عنوان کنترل مثبت، رسوب رنگ قهوه‌ای نشان دهنده حضور پروتئین سیناپتونمال کمپلکس…. 124
شکل 3- 30. تصویرمیکروسکوپی بیضه موش سوری بالغ به عنوان کنترل منفی.. 124
شکل 3- 31. تصویر میکروسکوپی تخمدان چپ جوجه تازه متولد شده شتر مرغ که با آنتیبادی Anti-SYCP1 و آنتیبادی ثانویه متصل به HRP مورد رنگآمیزی ایمونوهیستوشیمی قرار گرفته است. 125
شکل 3- 32. تصویر میکروسکوپی تخمدان جنین 36 روزه شتر مرغ که با Anti-SYCP1و آنتیبادی ثانویه متصل به HRP مورد رنگآمیزی ایمونوهیستوشیمی قرار گرفته است. 125
فهرست نمودارها
عنوان
نمودار 3- 1. مقایسه طول و عرض تخمدان چپ و راست در جنین و جوجه شتر مرغ.. 100
نمودار 4- 1. تغییر ابعاد تخمدان چپ و راست… 136
نمودار 4- 2. تغييرات حجم در تخمدان چپ جنين 20 روزه و جوجه تازه متولد شده شترمرغ.. 137
نمودار 4- 3. تعداد سلول‌های زایا در جوجه مرغ.. 138
نمودار 4- 4. تعداد سلول‌های زایا در شتر مرغ.. 138
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However, Video slots are the most common type of online slot. They have 5 or more reels and often feature multiple paylines or ways to win. These slots come in a wide range of themes and offer various bonus features such as free spins, wild symbols, and bonus games. The big real money prizes on tap make jackpot slots almost appealing…at least on the surface. But on average, progressive slots feature the lowest RTPs in gambling so you don’t have great winning odds. On top of that, you always need to bet the maximum to access the top jackpot prizes – meaning you might end up spending more money than you can afford to lose before you get even close to a win. You don’t even need to rely on blind trust, as Paddy Power only uses slots from trustworthy providers who have the appropriate gaming licences and whose games are fully tested by independent experts. You can feel certain every game is probably fair and you have a chance of winning on every spin that you play.
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Before depositing, always check if your desired online casino is licensed for the US. To legally offer online slot machines in the US, all sites need a valid license for the states where they operate. You should easily find a casino’s permit displayed on its website. If they do not have US licenses, we highly recommend that you avoid these sites. The current state regulators in the US are: Step 4: Choose your slot game: Online slot casinos offer a wide variety of games. Choose a slot game that fits your preferences and check its rules and paytable. As part of our ongoing evaluation of online casinos, we regularly test sites’ slots selection to produce our recommendations and rankings. For our pick of the best online casinos for high RTP slots, we considered a number of factors including:
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If you want to use your bronzer for a light all-over glow, use a fluffier powder brush for even coverage. If you’re new to doing a full face but want your contour to look like a pro did it, then reach for this Fenty Beauty brush. Its S-shaped angled head hugs your cheeks just right for a subtle contour that you can build up as you go. Take this quiz to find out. Hold brush at the center of handle or closer to the ferrule. Pinch brush handle between thumb and middle finger. Answer: Yes, you could definitely use a bronzer brush for blush but make sure to pick a round one. Round-shaped brushes are the best choice out of all the bronzer brushes to apply a little blush on your cheeks. Flat-top Kabukis or buffing brushes are great for applying foundation. Either works for both liquid and powder types. Use a them in a circular motion for totally even full coverage.