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بررسی تأثیر کميت­هاي تحريک الکتريکي با فرکانس پايين بر مهار روند صرع­زايي در کيندلينگ سریع مسير پرفورنت در موش صحرايي  

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بررسی تأثیر کميت­هاي تحريک الکتريکي با فرکانس پايين بر مهار روند صرع­زايي در کيندلينگ سریع مسير پرفورنت در موش صحرايي  

چكيده

هدف: يک نوع درمان جديد براي صرع مقاوم به دارو، تحريک الکتريکي مستقيم ناحيه صرعي توسط  تحريک الکتريکي با فرکانس پايين (low-frequency stimulation; LFS) می باشد. برای بررسی مکانيسم هاي اثرات ضد تشنجي LFS ، بهينه کردن کميت­هاي  LFSبه منظور ايجاد بيشترين کاهش در روند صرعي شدن  بسيار مهم مي باشد.در این تحقیق برخی از کمیت های LFS از قبیل تأثیر فرکانس، تعداد پالس، و فاصله زماني بين تحريکات کيندلينگ و اعمال LFS بر ميزان کاهش اثرات ضد تشنجی LFS  مورد مطالعه قرار گرفت.

مواد و روش ها:  در تمامی حیوانات الکترود  تحریک در مسیر پرفورنت و الکترود ثبات در شکنج دندانه دار قرار داده شد. یک گروه از حیوانات فقط تحریکات کیندل کننده را تا زمان کیندل شدن دریافت کردند و در گروه های دیگر الگوه های مختلف LFS به مدت 20 روز به دنبال پایان تحریکات کیندلینگ به حیوانات اعمال شد.

نتایج: در آزمایش اول تعداد پالس مورد آزمایش قرار گرفت. حیوانات به سه گروه  مختلف تقسیم شدند و 4 بسته ، 1 بسته و 8 بسته LFS ( هر بسته شامل 200 پالس یک میلی ثانیه ای با فرکانس 1 هرتز( را 5 دقیقه پس از اتمام تحریکات کیندلینگ دریافت کردند، گروهی که بیشترین اثر ضد تشنجی را داشت، گروهی بود که 4 بسته LFS دریافت می کرد بنابراین از این الگوی  LFSدر آزمایش بعد استفاده شد. در آزمایش دوم 4 بسته LFS در فواصل زمانی 30 ثانیه و 30 دقیقه پس از پایان تحریکات کیندلینگ اعمال شد. در گروهی که فاصله بین اتمام تحریکات و اعمال LFS 30 ثانیه بود، بیشترین اثر ضد تشنجی مشاهده گردید، بنابراین این الگوی LFS در آزمایش سوم مورد استفاده قرار گرفت. در آزمایش سوم تأثیر LFS در فرکانس های 25/0 و 5 هرتز مورد مطالعه قرار گرفت و گروهی که بیشترین اثر ضد تشنجی داشت گروهی بود که LFS  را با فرکانس  25/0 هرتز دریافت کرده بود.

نتیجه گیری: نتایج حاصله نشان داد که کمیت های مختلف LFS نقش مهمی در اعمال اثرات ضد تشنجی دارند. در این میان به نظر می رسد که هر چه فرکانس LFS کمتر و فاصله بین آن و تحریکات کیندل کمتر باشد اثر ضد تشنجی بیشتری خواهد داشت اما بین تعداد پالس های LFS و شدت اثر ضد تشنجی آن رابطه مستقیمی وجود ندارد.و تعیین پارامترهایی از LFS که بیشترین اثر ضد تشنجی را دارد می تواند در شناخت بهتر مکانیسم های درگیر در اثر ضد تشنجی LFS موثر باشد.

کلید واژگان: صرع، تحریک الکتریکی با فرکانس پایین، کیندلینگ

 

 

دسته: , برچسب: , ,

 

فهرست مطالب

مقدمه و  مروری بر مطالعات گذشته. 1

1-1مقدمه 2

1-2- صرع. 4

1-3- مدل صرعی كيندلينگ.. 4

1-3-1- مراحل رفتاري حملات تشنجي در مدل کيندلينگ.. 5

1-3-2- رابطه بين کيندلينگ و صرع در انسان. 6

1-3-3- مکانیسم ایجاد کیندلینگ.. 6

1-4- تشکيلات هيپوکمپ.. 7

1-4-1- شكنج دندانه دار و کيندلينگ.. 8

1-5- الكتروتراپي. 9

1-5-1- کاربرد الکتروتراپي در درمان صرع. 9

1-5-2- تحريک الکتريکی با فرکانس پايين. 10

1-5-3- اثرات LFS در کيندلينگ.. 11

1-6-1 تأثير تغييرات فركانس LFS بر اثر بخشي آن. 11

1-6-2 تاثیر اعمال LFS در بازه های زمانی متفاوت.. 12

1-7 هدف تحقيق. 12

فصل دوم. 14

مواد و روش ها 14

2-1- آماده سازي مواد و وسايل لازم 15

2-1-1- تهيه الكترود 15

2-2- جراحي حيوانات.. 16

2-3- تحريك حيوان. 16

2-4- كميت هاي اندازه گيری شده 18

2 -5- گروه هاي آزمايشي حيوانات به دو گروه کلي تقسيم میشدند: 18

2-6- روش تجزيه و تحليل آماري. 19

فصل سوم. 21

يافته ها  و نتايج. 21

3-1- نتایج. 22

فصل چهارم. 32

بحث، نتيجه گيری و پيشنهادها 32

1-4 – بحث و نتیجه گیری. 33

پیشنهادها 37

منابع. 38

 

 فهرست شکل ها:

شکل 1-1نمایی از ورودی و خروجی های شکنج دندار…………………………………………………………………………………………………………….8

شكل2-1:  دستگاههاي تحريك كننده وثبات ……………………………………………………………………………………………………………..17

شکل 2-2 نمایش دیاگرام زمانی انجام پروسه اعمال تحریکات کیندلینگ و LFS…………………………………………………………………19

شکل 3-1 تاثیر اعمال 1،4،8 بسته LFS بر مدت زمان تخلیه متعاقب روزانه طی روند کیندلینگ مسیر پرفورنت…………………………………………………………………………………………………………………………………………………………………………….23

شکل3-2 تاثیر اعمال 1،4،8 بسته LFS بر مدت زمان تخلیه متعاقب روزانه طی روند کیندلینگ مسیر پرفورنت در طی 20 روز……………………………………………………………………………………………………………………………………………………………………………….24

شکل3-3 تاثیر اعمال 1،4،8 بسته LFS بر بروز مراحل رفتاري تشنج طی روند کیندلینگ مسیر پرفورنت…………………………………………………………………………………………………………………………………………………………………………24

شکل3-4 تأثير اعمال 1،4،8 بسته LFS در بسته های مختلف بر بروز مراحل رفتاري تشنج طي روند کيندلينگ مسير پرفورنت در طی 20 روز.…………………………………………………………………………………………………………………………………………………………………….25

شکل3-  5تاثیر اعمال LFS در فواصل متفاوت پس از پایان تحریکات کیندلینگ……………………………………………………………………………………………………………………………………………………………………………26

شکل3- 6تاثیر اعمال LFS در فواصل متفاوت پس از پایان تحریکات کیندلینگ…………………………………………………………………………………………………………………………………………………………………………..26

شکل3-7 تأثير اعمال LFS  در فاصله های مختلف پس از پایان تحریکات کیندلینگ بر بروز مراحل رفتاري تشنجطي روند کيندلينگ مسيرپرفورنت.……………………………………………………………………………………………………………………………………………………..27

شکل3-8. تأثير  اعمال LFS  در فاصله های مختلف پس از پایان تحریکات کیندلینگ بر بروز مراحل رفتاري تشنج طي روند کيندلينگ مسير پرفورنت در طی 20 روز…………………………………..…………………………………………………………………………………………….28

شکل3-9. تأثير  اعمال LFS  در فاصله های مختلف پس از پایان تحریکات کیندلینگ بر مجموع مراحل تشنجی در هر روز پس از هر تحریک کیندلینگ طي روند کيندلينگ مسير پرفورنت در 20 روز.…………………………………………………………………………………………….29

شکل3-10 تأثير  اعمال LFS با فرکانس های مختلف طي روند کيندلينگ مسير پرفورنت………………………………………………………………………………………………………………………………………………………………………………….29

شکل3- 11تأثير  اعمال LFS با فرکانس های مختلف طي روند کيندلينگ مسير پرفورنت.……………………………………………………………………………………………………………………………………………………………………………..30

شکل3-12 تاثير  اعمال LFS با فرکانس های مختلف طي روند کيندلينگ مسير پرفورنت بر بروز مراحل رفتاری تشنج……………………………………………………………………………………………………………………………………………………………………………. 31

شکل 3- 13تأثير  اعمال LFS با فرکانس های مختلف طي روند کيندلينگ مسير پرفورنت بر بروز مراحل رفتاری تشنج……………………………………………………………………………………………………………………………………………………………………………….31

 

 

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