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بررسي سایتوتوكسيسيتي خمیر پروفیلاکسی پریومیکس (PerioMix) در محیط کشت ردههای سلولی HGF و L929
تعداد 77 صفحه فایل word قابل ویرایش
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چكيده:
هدف:
خمیرهای پروفیلاکسی که در دندانپزشکی بعد از جرمگیری و تمیزکردن دندانها و یا قبل از جراحی های پریودنتال استفاده میشوند در جهت مراقبتهای پیشگیری و سلامت دهان بوده و همچنین کارآیی مناسبی در برداشتن رنگدانه (Stain)، پلاک میکروبی و همچنین حساسیتزدایی از دندانها میباشد. هر خمیر پروفیلاکسی بایستی از نظر بیولوژیکی و اثرات سمی احتمالی آن بر بافتهای دهانی و دندانی مورد آزمایش قرار گیرد و درصورت انطباق بیولوژیکی می تواند به طور کلینیکی مورد استفاده قرار گیرد.
در این مطالعه ما به بررسی سایتوتوکسیسیته (سمیت سلولی) خمیر پروفیلاکسی پريوميكس که ساخت هیئت علمی دانشکده مشهد بوده میپردازیم.
مواد و روش کار:
در اين مطالعه براي سنجش سميت خمير پريوميكس از دو روش سنجش كيفي توسط ميكروسكوپ نوري و سنجش كمي توسط تست MTT انجام گرديد. دو نوع محيط كشت HGF و L929 مورد استفاده قرار گرفت. در تست كيفي خمير پريوميكس را به مدت 10 دقيه در دو محيط كشت قرار داده و پس از برداشتن خمير در فواصل زماني بلافاصله، بعد از يك ساعت، بعد از 24 ساعت در زير ميكروسكوپ مشاهده كرديم. در تست MTT ميزان جذب پس از سه ساعت و 24 ساعت خوانده شد.
نتایج:
در بررسی کیفی توسط میکروسکوپ نوری سه ناحیه قابل مشاهده میباشد. ناحیه اول که در تماس مستقیم با خمیر میباشد، سلولها از سطح کشت کاملاً جدا شدهاند و ناحیهای عاری از هر گونه سلول دیده میشود. در ناحیه دوم که در مجاورت با خمیر میباشد تعداد سلولهای از بین رفته زیادی دیده می شود که به صورت ناحیهای پر رنگ در مجاورت ناحیه اول مشاهده گردید، در ناحیه سوم که فاصله دورتری از خمیر داشت سلولهای زنده بیشتری بود. مشاهدات پس از یک ساعت و 24 ساعت نشان داد که تغییر قابل ملاحظهای در وضعیت سلولها اتفاق نیافتاده است.
در بررسی کمی پس از سه ساعت میزان جذب نمونه کنترل 344/0 OD= بوده و نمونه با خمير به 198/0OD= كاهش يافته است و نشان ميدهد كه كمتر از %50 سلولها از بين رفتهاند. همين مورد در نمونه L929 ميزان مرگ و مير كمتر از %60 را نشان ميدهد. نتايج پس از 24 ساعت تغيير چنداني را نشان نميدهد.
بحث و نتيجهگيري:
با توجه به نتايج بدست آمده در بررسي كمي و كيفي همگي حاكي از سمي بودن خمير پريوميكس را دارد ولي چون مقدار آن كمتر از %50 ميباشد لذا ميتوان گفت كه ميزان سميت قابل ملاحظه نيست.
اين سميت ميتواند عوامل متفاوتي داشته باشد كه از جمله به علت اندازه ذرات كه در حد نانومتر ميباشد و يا طبيعت سمي مواد به كار رفته از جمله اكسيد سيليسيوم و اكسيد آلومينيوم در خمير ميباشد. از طرف ديگر چون خمير مورد نظر در كلينيك و فقط يكبار استفاده ميشود لذا ميتوان نتيجهگيري كرد كه ميزان سميت نميتواند قابل توجه باشد.
واژگان كليدي: سميت، خمير پريوميكس، سلول HGF و L929، تست MTT
دسته: دندانپزشکی, علوم تجربی
برچسب: تست MTT, خمير پريوميكس, سلول HGF و L929, سميت
39 دیدگاه برای بررسي سایتوتوكسيسيتي خمیر پروفیلاکسی پریومیکس (PerioMix) در محیط کشت ردههای سلولی HGF و L929
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